ELISA Methods Based on Monoclonal Antibodies for the Serological Diagnosis of Lumpy Skin Disease

A Capture ELISA for the Diagnosis of Visceral Leishmaniasis Using a Monoclonal Antibody against a Leishmanial

capture ELISA system was developed for diagnosis of visceral leishmaniasis (VL) using a monoclonal antibody raised against an antigen previously detected in the urine of VL patients. Urine samples from confirmed VL cases from Yemen, Nepal, Spain, Sudan and Brazil were tested in the capture ELISA in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...

Lumpy Skin Disease

Lumpy skin disease is a poxviral disease with significant morbidity in cattle. Although the mortality rate is generally low, economic losses result from loss of condition, decreased milk production, abortions, infertility and damaged hides. The causative virus seems to be spread mainly by insects, and outbreaks can be widespread and difficult to control. Lumpy skin disease was confined to Afric...

Lumpy Skin Disease

Lumpy skin disease (LSD, knopvelsiekte) is a pox disease of cattle characterised by fever, nodules 4 on the skin, mucous membranes and internal organs, emaciation, enlarged lymph nodes, oedema 5 of the skin, and sometimes death. The disease is of economic importance because it causes 6 reduced production, particularly in dairy herds. It also causes damage to the hide. LSD is caused by 7 strains...

Measurement of Affinity Constant of Anti-human IgG Monoclonal Antibodies by an ELISA-based Method

Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

Lumpy skin disease